Table 1. Summary of protein–lipid interactions selected for validation.
| Protein name | Lipid | Validation assay | |
|---|---|---|---|
| aProtein–lipid interactions that could not be confirmed. | |||
| Liposomes, binding to liposomes; mss4ts, perturbation of cellular PtdInsPs using an mss4ts mutant; myriocin, perturbation of cellular sphingolipids using myriocin. | |||
| Ecm25 | PtdIns | Liposomes | |
| Ecm25 | PtdIns(3,4,5)P3 | Liposomes | |
| Ira2 | PtdIns(3,4,5)P3 | Liposomes | |
| Las17 | PtdIns(3,5)P2 | Liposomes/mss4ts | |
| Las17 | PtdIns(3,4,5)P3 | Liposomes | |
| Pkh2 | PtdIns | Liposomes | |
| Pkh2 | PtdIns(4)P | Liposomes/mss4ts | |
| Pkh2 | PtdIns(3,4)P2 | Liposomes | |
| Pkh2 | PtdIns(3,5)P2 | Liposomes/mss4ts | |
| Pkh2 | PtdIns(4,5)P2 | Liposomes/mss4ts | |
| Slm1 | PtdIns(4,5)P2 | Liposomes/mss4ts | |
| Rvs167 | PtdIns(4,5)P2 | Liposomes/mss4ts | |
| Slm1 | DHS-1P | Liposomes/myriocin | Pkh1/2 independent |
| Rvs167 | DHS-1P | Liposomes/myriocin | Pkh1/2 independent |
| Slm1 | PtdIns(4)P | mss4ts | |
| Rvs167 | PtdIns(4)P | mss4ts | |
| Rvs161 | PtdIns(3)P | mss4ts | |
| Ypk1 | PtdIns(4)P, PtdIns(4,5)P2 | mss4ts | |
| Ecm25a | PHS-1P, Sph, Sph-1P | Myriocin | |
| Las17 | PHS-1P, Sph-1P | Myriocin | Pkh1/2 independent |
| Mss4a | DHS-1P | Myriocin | Pkh1/2 dependent |
| Rvs167 | PHS-1P | Myriocin | Pkh1/2 independent |
| Ypk1 | DHCer, PHCer-C18 | Myriocin | Pkh1/2 independent |
| Spo14a | PHS, Sph, DHCer, PHCer-C8, PHCer-C18, Cer | Myriocin | |