Table 2. Growth on plates containing M9/glucose at 37°C of strains lacking various enzymes in the PLP synthesis pathway when the indicated genes are overexpressed from pTrcHisB.
| Gene | ΔpdxB (JU283) | ΔpdxB (JU283)+suppl.a | ΔserC (JWK0890)+suppl.a | ΔpdxA (JWK0051) | ΔpdxJ (JWK2548) | ΔpdxH (JWK1630) |
|---|---|---|---|---|---|---|
| Strain designations are defined in Supplementary Table I. | ||||||
| aL-Ser (1.0 mM), L-Phe (0.1 mM), L-Tyr (0.1 mM), L-Trp (0.1 mM), p-hydroxybenzoate (1 μM), p-aminobenzoate (1 μM) and 2,3-dihydroxybenzoate (1 μM). | ||||||
| bColonies reached 1 mm diameter in 1–2 days (++++), 3–5 days (+++) or 6–8 days (++). (+) indicates colonies that were <1 mm after 9 days. | ||||||
| pdxB | ++++ b | ++++ | − | − | − | − |
| hisB | ++++ | ++++ | − | − | − | − |
| yjbQ | +++ | +++ | − | − | − | − |
| php | ++++ | ++++ | − | − | − | − |
| thrB | ++++ | + | − | − | − | − |
| yeaB | +++ | + | − | − | − | − |
| pdxA | +++ | ++ | − | ++++ | − | − |
| aroB | +++ | + | − | ++ | − | − |