Table 3. Kinetic parameters for the activities of enzymes that can catalyze reactions in pathway 1.
| Protein | Substrate | kcat (s−1) | KM (mM) | kcat/KM (M−1 s−1) |
|---|---|---|---|---|
| Substrates involved in pathway 1 are highlighted in bold. | ||||
| aYeaB was expressed as a fusion protein with maltose-binding protein (MalE) to improve its solubility. | ||||
| b(Kuzuyama et al, 2000). | ||||
| MalE–YeaBa | 3PHP | 5.7±0.7 × 10−5 | <2 | >0.028±0.003 |
| Dxs | Pyruvate+D-GAPb | 345 | 0.096 (pyruvate) | 3.6 × 106 |
| 0.24 (D-GAP) | 1.4 × 106 | |||
| 3HP | 0.026±0.001 | 0.050±0.008 | 5.2±0.4 × 102 | |
| SucA | 3HP | 0.0139±0.0003 | 0.72±0.07 | 19.3±1.9 |
| LtaE | L-allo-threonine | 3.2±0.2 | 0.052±0.004 | 6.2±0.5 × 104 |
| L-threonine | 1.1±0.1 | 4.0±0.2 | 2.8±0.2 × 102 | |
| 4HT | 1.44±0.03 | 0.027±0.002 | 5.3±0.4 × 104 | |
| ThrB | Homoserine | 46±4 | 0.12±0.02 | 3.8±0.7 × 105 |
| 4HT | 9.7±0.8 | 2.0±0.1 | 4.8±0.6 × 103 | |