Table 4. The effect of serine and homoserine on complementation of the ΔpdxB, ΔpdxB ΔserA and ΔpdxB serA’ strains by overexpression of pdxB or yeaB.
| Supplementsa | Strain and overexpressed gene |
|||||
|---|---|---|---|---|---|---|
| ΔpdxB (JU283) |
ΔpdxB ΔserA (JU425) |
ΔpdxB serA’ (JU430) |
||||
| pdxB | yeaB | pdxB | yeaB | pdxB | yeaB | |
| Strain designations are defined in Supplementary Table 1. | ||||||
| aL-serine concentration was 1 mM and L-homoserine concentration was 50 μM. | ||||||
| bColonies reached 1 mm diameter in 1–2 days (++++), 3–5 days (+++) or 6–8 days (++). (+) indicates colonies that were <1 mm after 9 days. | ||||||
| None | ++++b | +++ | − | − | ++++ | ++ |
| L-serine | ++++ | + | +++ | − | +++ | − |
| L-serine+L-homoserine | ++++ | + | +++ | − | ++++ | ++ |