Table 1.
Proteins identified by in-gel digestion and nanoLC-MS/MS of SDS-PAGE-isolated bands
Accession number | Protein description | Theoretical Mw | No. unique peptides | Probability | Mascot score* | Sequence coverage |
---|---|---|---|---|---|---|
IPI00022434 | ALB Uncharacterized protein | 71.6 | 45 | 1 | – | 67.5 |
IPI00021841 | ALB apoliprotein a1 | 30.8 | 25 | 1 | – | 72.0 |
IPI00021885 | FGA Isoform 1 of Fibrinogen alpha chain precursor | 94.9 | 15 | 1 | – | 19.7 |
IPI00304273 | APOA4 Apolipoprotein A-IV precursor | 45.3 | 13 | 1 | – | 34.1 |
IPI00022371 | HRG Histidine-rich glycoprotein precursor | 59.5 | 11 | 1 | – | 24.8 |
IPI00553177 | Isoform 1 of Alpha-1-Antitrypsin | 46.7 | 10 | 1 | – | 24.2 |
IPI00021842 | APOE Apolipoprotein E precursor | 36.1 | 10 | 1 | – | 36.0 |
IPI00783987 | C3 Complement | 187.0 | 9 | 1 | – | 4.5 |
IPI00399007 | IGHG2 Putative uncharacterized protein DKFZp686I04196 (Fragment) | 46.0 | 5 | 1 | – | 14.1 |
IPI00419424 | IGKV1-5 IGKV1-5 protein | 26.2 | 5 | 1 | – | 32.5 |
IPI00855916 | Transthyretin | 15.9 | 4 | 1 | – | 48.0 |
IPI00021855 | APOC1 Apolipoprotein C1 | 9.3 | 3 | 0.996 | – | 24.0 |
IPI00032258 | Complement c4 | 192.6 | 3 | 1 | – | 2.5 |
IPI00061977 | IGHV3OR16-13;IGHA1 IGHA1 protein | 54.1 | 2 | 0.999 | – | 5.0 |
IPI00022488 | HPX Hemopexin precursor | 51.6 | 2 | 0.983 | – | 4.1 |
IPI00154742 | IGL@ IGL@ protein | 24.7 | 2 | 0.999 | – | 10.7 |
IPI00019399 | SAA4 Serum amyloid A-4 protein precursor | 14.8 | 2 | 0.998 | – | 8.5 |
IPI00298971 | VTN Vitronectin precursor | 54.2 | 2 | 0.995 | – | 5.4 |
IPI00021857 | APOC3 Apolipoprotein C3 | 12.8 | 1 | 123 | 16.0 | |
IPI00021856 | APOC2 Apolipoprotein C-II precursor | 11.3 | 1 | 42 | 10.0 | |
IPI00431645 | HP protein | 31.6 | 1 | 30 | 9.0 | |
IPI00019038 | LYZ Lysozyme C precursor | 16.9 | 1 | 73 | 8.0 | |
IPI00026314 | GSN Isoform 1 of Gelsolin precursor | 86.0 | 1 | 27 | 1.0 | |
IPI00298497 | FGB Fibrinogen beta chain precursor | 56.5 | 1 | 43 | 3.0 | |
IPI00032328 | KNG1 Isoform HMW of Kininogen-1 precursor | 72.3 | 1 | 34 | 1.0 |
Notes: In gray, protein identification obtained with a single hit. Those hits were validated by visual inspection. MS/MS data are reported in Supplementary Information. Mascot score (*) is reported for identifications which have not passed TPP validation, but were above Mascot threshold of 29 and for which manual validation was undertaken.