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. 2010 Dec 20;124(2):198–206. doi: 10.1242/jcs.072652

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Fig. 1. — The IL domain of Cx50 is sufficient in mediating the interaction with AQP0. (A) Schematic representation of chimeric connexins with swapped IL domains. Cx50*46L was generated by fusing Cx50 lacking the IL domain with the IL from Cx46. Similarly, Cx50*43L was generated by fusing Cx50 lacking the IL domain with the IL from Cx43; Cx43*50L was generated by fusing Cx43 lacking the IL domain with the IL from Cx50, and Cx43*46L was generated by fusing Cx43 lacking the IL domain with the IL from Cx46. (B) Recombinant retroviruses containing RCAS(A) vehicle (lane 1), Cx50 (lane 2), Cx46 (lane 3), Cx43 (lane 4), Cx50*46L (lane 5), Cx50*43L (lane 6), Cx43*50L (lane 7) and Cx43*46L (lane 8) were infected into CEF cells. Six days after infection, cell lysates were analyzed by SDS-PAGE and immunoblotted with antibodies against FLAG (upper panel) or β-actin (middle panel). Glutathione agarose beads conjugated with GST-tagged AQP0(CT) were used to pull down proteins from the lysates of infected CEF cells. The corresponding eluted fractions were immunoblotted with anti-FLAG antibody (bottom panel).