Fig. 6.

The activity of hemichannels formed by Cx50, Cx46 or chimeric mutants is not affected by AQP0. CEF cells infected with recombinant retroviruses containing RCAS(A), AQP0, Cx50, Cx46, Cx50*46L or various combinations were cultured at low cell density with minimal physical contact. After applying mechanical stimulation through fluid dropping, the numbers of cells with LY or RD dye uptake were counted under a fluorescence microscope with non-dropping groups as a negative control. The extent of dye uptake was quantified as a percentage of fluorescent cells divided by the total number of cells. The data are presented as mean ± s.e.m. (n=3).