Table 1.

Literature values of diffusion coefficients in water at 25 °C

Protein	D w a (×10−6 cm2/s)	r H b (nm)	MW (×103)
Bovine chymotrypsinogen72	0.71	3.5	24.0
BSA97	0.72	3.4	66.5
Bovine hemoglobin97	0.84	2.9	68.0
Brain-derived neurotrophic factor89	0.37	6.6	27.3
Epidermal growth factor 93	1.3	1.9	6.60
Human immunoglobulin G65	0.38	6.5	146
Human immunoglobulin M65	0.18	14	970
NGF90	1.0	2.4	130
Porcine insulin97	1.3	1.8	5.80
VEGF 16517	0.52	4.7	19.0

^aValues adjusted for temperature using Eq. (2)

^bCalculated using Eq. (1)