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. Author manuscript; available in PMC: 2011 May 28.
Published in final edited form as: Biochem Biophys Res Commun. 2010 May 8;396(2):562–565. doi: 10.1016/j.bbrc.2010.04.148

Fig. 1.

Fig. 1

HDM2 levels are suppressed by preventing PA production. 786-O cells were plated at 70% confluence in 60mm plates in 10% serum and allowed to grow overnight. 24 hrs later, the cells were either left untreated (nt) or treated with 0.8% 1-BtOH or 0.8% t-BtOH as a negative control for 3 hrs. The cells were then lysed and subjected to Western blot analysis with antibodies that recognize HDM2 or actin as a loading control.