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Knockdown of mTORC2 and not mTORC1 results in loss of HDM2. 786-O cells were plated at 30% confluence in 6-well plates. The cells were transfected with siRNA for Raptor, mTOR, or Rictor for 48hrs. As controls cells were left not treated (nt), treated with reagent only (re), or transfected with scrambled siRNA (scr). Actin was used as a loading control. The data are representative of three independent experiments.
