Skip to main content
. Author manuscript; available in PMC: 2011 May 28.
Published in final edited form as: Biochem Biophys Res Commun. 2010 May 8;396(2):562–565. doi: 10.1016/j.bbrc.2010.04.148

Fig. 3.

Fig. 3

Knockdown of AGC member SGK1 but not Akt1 or Akt2 results in loss of HDM2. (A) 786-O cells were plated at 30% confluence in 6-well plates. The cells were transfected with siRNA for Akt1 and Akt2 for 48hrs. (B) 786-O cells were plated as in A and then transfected with siRNA for SGK1 for 48hrs. As controls wells were left untreated (nt), treated with reagent (re), or transfected with scrambled siRNA (scr). Actin was used as a loading control. The blots are representative of at least three independent experiments.