Fig. 1. Myxothiazol and oligomycin collapse mitochondrial membrane potential in HepG2 cells.
Cells in HBSS were loaded with TMRM, as described in MATERIALS AND METHODS. After collecting baseline images, myxothiazol (10 μM) was added, and another image was collected after 20 min. Oligomycin (10 μg/ml) was then added and additional images were collected after 30 min. In upper panels, image intensity is pseudocolored according to the reference bar. Lower panels are brightfield images.