Figure 4.

Equilibrium analyses of five lower-affinity β₁AR analytes. Each compound was tested in duplicate in a two-fold dilution series starting at 100uM. Responses at equilibrium (t = 10–20 sec) were plotted against compound concentration and fit to a simple binding isotherm. Analyte structures are shown in the insets; compound identities and affinities from the isotherms are listed in Table 1.