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cpTi particles with adherent PAMPs do not induce the TLRs to associate with detergent rich membranes (DRMs) isolated by sucrose gradient flotation. RAW264.7 cells were incubated with PBS, cpTi particles with adherent PAMPs, or LPS for 60 min prior to lysis, sucrose gradient fractionation, and Western blotting for TLR4, TLR2, and the lipid raft marker CD-14.
