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“As received” E. coli and the supernatant obtained by centrifugation induce TLR-4 to associate with DRMs but centrifuged E. coli particles do not. RAW264.7 cells were incubated with the stimuli for 60 min prior to lysis, sucrose gradient fractionation, and Western blotting for TLR4, TLR2, and the lipid raft marker CD-14.
