Table 3.
entry | pyrazoline | λabs (nm) | ε (M−1 cm−1) | λem (nm)[b] | φF[c] |
---|---|---|---|---|---|
1 | 2b | 358 | 20,356 | 456 | 0.30 |
2 | 3b | 364 | 10,190 | 466 | 0.35 |
3 | 4b[d] | 348 | 1,702 | 468 | 0.35 |
4 | 5b[d] | 348 | 830 | 464 | 0.17 |
5 | 6b | 358 | 13,852 | 451 | 0.32 |
6 | 9b | 376 | 19,440 | 477 | 0.25 |
7 | 10b | 350 | 12,534 | 502 | 0.15 |
Compounds were dissolved in PBS:ACN (1:1) at the concentrations of 5 μM unless noted otherwise.
λex = 348 nm.
Quantum yields were determined using DAPI as the standard (φF = 0.58 in DMSO).
20 μM concentrations were used.