Table 2. Metabolite changes in plasma at the peak exercise time point.

Metabolites with P<0.005 in the derivation cohort are shown. Metabolites with identical retention times and parent-daughter ion pairs (e.g. isoleucine and leucine) cannot be distinguished by the platform

Metabolite	Derivation Cohort N= 45 % CHANGE (IQR)	P value	Validation Cohort N=30 % CHANGE (IQR)	P value
Lactate	394 (263, 697)	<0.0001	553 (332, 697)	<0.0001
Malate	120 (63, 232)	<0.0001	143 (96, 203)	<0.0001
Succinate	246 (125, 379)	<0.0001	345 (217, 492)	<0.0001
Glycerol	59 (31, 126)	<0.0001	50.8 (25, 117)	0.0001
Fumarate	56 (31, 83)	<0.0001	75 (47, 133)	<0.0001
Pyruvate	169 (78, 308)	<0.0001	169 (96, 289)	<0.0001
Niacinamide	79 (42, 182)	<0.0001	71(11, 148)	0.0005
Pantothenate	22 (1, 64)	<0.0001	29 (9, 86)	0.0003
Glucose-6-phosphate	20 (34, 46)	<0.0001	25 (11, 74)	0.002
Alanine	29 (-1, 49)	<0.0001	30 (3, 41)	0.0008
Inosine	83 (7, 181)	<0.0001	68 (15, 250)	0.0005
Hypoxanthine	87 (31, 201)	<0.0001	132 (68, 295)	<0.0001
Citrulline	-8 (-19, -0.6)	0.0003	-12 (-19, -2.8)	0.021
AMP	37(-10, 75)	0.0002	56 (10, 125)	0.004
Isoleucine/Leucine	3(-1, 10)	0.0005	9 (3, 17)	0.0005
Serine	-14 (-25, -5)	0.0006	-11 (-22, -3)	0.0061
Glutamate	-13 (-27, 0)	0.001	-10 (-30, 16)	0.21
Xanthine	18 (-5.0, 46)	0.002	14 (7, 66)	0.002
Cysteine	-19 (-34.5, 8.2)	0.002	-22 (-36, -15.0)	0.0001
Allantoin	-28 (-44, -7.4)	0.003	-15 (-28, 2.5)	0.02
3-Phosphoglycerate	26 (-5, 43)	0.0035	34 (-12, 67)	0.06
Homocysteine	-14 (-21, 4.2)	0.0039	-15 (-24, 2.6)	0.022
Glutamine	8 (1, 17)	0.004	6 (-4, 14.3)	0.046