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. Author manuscript; available in PMC: 2010 Dec 27.
Published in final edited form as: Nat Genet. 1998 Apr;18(4):325–330. doi: 10.1038/ng0498-325

Fig. 4.

Fig. 4

Gene targeting by transduction and transfection. HSNO39 cells were either transduced with the AAV-SNO648 vector at a MOI of 4 × 105 vector genomes per cell or transfected with pASNO648 or pASNori2 plasmids, and the fraction of G418-resistant CFU was determined. Transfections were performed by plating 4 × 106 cells per 15-cm dish on day 1, changing the media and transfecting with 20 µg of the indicated plasmid as a calcium-phosphate precipitate on day 2, treating with trypsin and replating dilutions of cells on day 3, and selecting with G418 on day 4 (as in the transduction experiments). pASNO648 was linearized by digestion with SacII before transfection. Mean values with standard errors are plotted.