Figure 3.
Western analysis of APE1/Ref-1 expression in the retina, RVEC and RPC. High levels of APE1/Ref-1 protein were detected by a monoclonal anti-APE1/Ref-1 antibody in the retinal tissue and in purified retinal vascular cells, RVEC and RPC from wild-type (+/+) and Vldlr−/− (−/−) mice. APE1/Ref-1 expression level was similar in the two mice strains. Anti-tubulin antibody was used as an internal control. The result is representative of 3 replicates.