Table 1. Identified proteins related to UPR/ER stress pathways following treatment with rotenone.
Proteins listed with International Protein Index (IPI) [16], MASCOT score, and function. Protein not described in text listed with references to PD and neurodegeneration.
| Rotenone 48 hrs | Function | Protein | Accession Numbers | Mascot Score |
|---|---|---|---|---|
| Control | chaperone/foldase | HSP90-beta [5,14] | IPI00414676 | 422 |
| chaperone/foldase | HSP90-alpha isoform 2 [5,14] | IPI00382470 | 288 | |
| chaperone/foldase | GRP75 | IPI00007765 | 90 | |
| chaperone/foldase | HSP60, mitochondrial | IPI00784154 | 151 | |
| chaperone/foldase | TCP1 T-complex subunit 3 isoform b [38] | IPI00290770 | 67 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit alpha [38] | IPI00290566 | 75 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit beta [38] | IPI00297779 | 202 | |
| chaperone/foldase | Peptidyl-prolyl cis-trans isomerase A | IPI00419585 | 40 | |
| UPS system | Ubiquitin carboxyl-terminal hydrolase isozyme | IPI00018352 | 192 | |
| UPS system | Ubiquitin-like modifier-activating enzyme 1 | IPI00645078 | 127 | |
| antioxidant | Glutathione S-transferase | IPI00219757 | 132 | |
| 5nM | chaperone/foldase | HSP90-beta [6,15] | IP00I414676 | 155 |
| chaperone/foldase | HSP60, mitochondria | IPI00784154 | 101 | |
| chaperone/foldase | HSP75, mitochondria | IPI0030275 | 65 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit zeta [38] | IPI00027626 | 53 | |
| chaperone/foldase | Peptidyl-prolyl cis-trans isomerase A | IPI00419585 | 63 | |
| chaperone/foldase | Prefoldin subunit 5 | IPI00015361 | 93 | |
| UPS system | Ubiquitin-like modifier activating enzyme 1 | IPI00552452 | 33 | |
| UPS system | Proteasome subunit beta type 3 | IPI00028004 | 88 | |
| antioxidant | Glutathione S-Transferase | IPI00219757 | 34 | |
| antioxidant | Peroxiredoxin-2 [9] | IPI00027350 | 31 | |
| mitochondrial maintenance | Prohibitin-2 [22] | IPI00027252 | 59 | |
| modulates oxidative stress | Protein DJ-1 | IPI00298547 | 40 | |
| 10nM | chaperone/foldase | HSP90-beta [5,14] | IPI00414676 | 241 |
| chaperone/foldase | HSP90AA1 isoform 1 [5,14] | IPI00784295 | 77 | |
| chaperone/foldase | HSP90AB4P [5,14] | IPI00555565 | 49 | |
| chaperone/foldase | BiP | IPI00003362 | 97 | |
| chaperone/foldase | HSP70 protein 6 [25] | IPI00339269 | 45 | |
| chaperone/foldase | HSC71 isoform 1[25] | IPI00003865 | 130 | |
| chaperone/foldase | GRP75 | IPI00007765 | 53 | |
| chaperone/foldase | HSP60, mitochondrial | IPI00784154 | 29 | |
| chaperone/foldase | HSP90 co-chaperone Cdc37 | IPI00013122 | 70 | |
| chaperone/foldase | HSP70/HSP90 organizing protein [4] | IPI00013894 | 29 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit beta [38] | IPI00297779 | 116 | |
| chaperone/foldase | Peptidyl-prolyl cis-trans isomerase A [36] | IPI00419585 | 44 | |
| chaperone/foldase | Protein disulfide-isomerase A6 isoform 2 [36] | IPI00299571 | 89 | |
| chaperone/foldase | Protein disulfide-isomerase A3 [36] | IPI00025252 | 38 | |
| chaperone/foldase | Prefoldin subunit 6 | IPI00005657 | 46 | |
| UPS system | Ubiquitin carboxyl-terminal hydrolase isozyme | IPI00018352 | 51 | |
| UPS system | Ubiquitin-like modifier-activating enzyme 1 | IPI00645078 | 46 | |
| UPS system | Ubiquitin-conjugating enzyme E2 | IPI00003949 | 57 | |
| UPS system | 26S proteasome subunit S10B protease | IPI0021926 | 43 | |
| UPS system | 26S proteasome subunit B5 isoform 3 | IPI00383971 | 29 | |
| antioxidant | Glutathione S-transferase | IPI00219757 | 152 | |
| antioxidant | Superoxide dismutase | IPI00218733 | 58 | |
| modulates oxidative stress | Protein DJ-1 | IPI00298547 | 62 | |
| 20nM | chaperone/foldase | HSP90-beta [5,14] | IPI00414676 | 347 |
| chaperone/foldase | HSP90-alpha isoform 1 [5,14] | IPI00784295 | 107 | |
| chaperone/foldase | HSP90B1 endoplasmin/GRP94 [5,14] | IPI00027230 | 73 | |
| chaperone/foldase | BiP | IPI00003362 | 77 | |
| chaperone/foldase | HSC71 isoform 1 [25] | IPI00003865 | 329 | |
| chaperone/foldase | HSP70 protein 7 [25] | IPI00011134 | 109 | |
| chaperone/foldase | GRP75 | IPI00007765 | 58 | |
| chaperone/foldase | HSP60, mitochondrial | IPI00784154 | 258 | |
| chaperone/foldase | HSP27 [24,28] | IPI00025512 | 31 | |
| chaperone/foldase | TCP1 T-complex subunit 3 isoform b [38] | IPI00290770 | 63 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit alpha [38] | IPI00290566 | 91 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit beta [38] | IPI00297779 | 52 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit delta [38] | IPI00302927 | 72 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit epsilon [38] | IPI00010720 | 110 | |
| chaperone/foldase | Peptidyl-prolyl cis-trans isomerase A | IPI00419585 | 109 | |
| chaperone/foldase | Peptidyl-prolyl cis-trans isomerase B | IPI00646304 | 60 | |
| chaperone/foldase | Protein disulfide-isomerase A6 isoform 2 [36] | IPI00299571 | 73 | |
| chaperone/foldase | Protein disulfide-isomerase A3 [36] | IPI00025252 | 70 | |
| chaperone/foldase | Calreticulin | IPI00020599 | 38 | |
| UPS system | Ubiquitin-conjugating enzyme E2 | IPI00003949 | 69 | |
| UPS system | Proteasome subunit alpha type-1 | IPI00016832 | 49 | |
| UPS system | Proteasome subunit alpha type-7 | IPI00024175 | 40 | |
| antioxidant | Glutathione S-transferase | IPI00246975 | 41 | |
| antioxidant | Superoxide dismutase | IPI00218733 | 92 | |
| antioxidant | Peroxiredoxin-1 [9] | IPI00000874 | 48 | |
| antioxidant | Peroxiredoxin-6 [9] | IPI00220301 | 122 | |
| mitochondrial maintenance | Prohibitin-1 [22] | IPI00017334 | 59 | |
| mitochondrial maintenance | Prohibitin-2 [22] | IPI00027252 | 78 | |
| microtubule remodeling | Stathmin | IP00479997 | 104 | |
| microtubule remodeling | Stathmin-2 | IPI00218667 | 49 | |
| microtubule remodeling | Stathmin-4 isoform 1 | IPI00006575 | 41 | |
| export misfolded proteins | Transitional ER ATPase | IPI00022774 | 38 | |
| antinflammatory | Cytosolic Phosolipase A2 | IPI00384577 | 54 | |
| 50nM | chaperone/foldase | HSP90-beta [5,14] | IPI00414676 | 266 |
| chaperone/foldase | HSP90-alpha isoform 2 [5,14] | IPI00382470 | 173 | |
| chaperone/foldase | HSP90B1 endoplasmin/GRP94 [5,14] | IPI00027230 | 32 | |
| chaperone/foldase | BiP | IPI00003362 | 71 | |
| chaperone/foldase | HSP75, mitochondrial | IPI00030275 | 77 | |
| chaperone/foldase | HSP70 protein 1 [25] | IPI00304925 | 30 | |
| chaperone/foldase | HSP70 protein 6 [25] | IPI00339269 | 23 | |
| chaperone/foldase | HSC71 isoform 1 [25] | IPI00003865 | 103 | |
| chaperone/foldase | HSP60, mitochondrial | IPI00784154 | 182 | |
| chaperone/foldase | HSP10, mitochondrial [11] | IPI00220362 | 78 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit alpha [38] | IPI00290566 | 45 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit 3 isoform c [38] | IPI00552715 | 54 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit zeta [38] | IPI00027626 | 34 | |
| chaperone/foldase | Peptidyl-prolyl cis-trans isomerase A | IPI00419585 | 36 | |
| chaperone/foldase | Peptidyl-prolyl cis-trans isomerase B | IPI00646304 | 52 | |
| chaperone/foldase | ERp57 [7] | IPI0025252 | 51 | |
| chaperone/foldase | Protein disulfide isomerase A5 [36] | IPI0031479 | 51 | |
| UPS system | Ubiquitin carboxyl-terminal hydrolase isozyme L1 | IPI00018352 | 62 | |
| UPS system | E3 Ubiquitin ligase UBR5 | IPI00026320 | 35 | |
| UPS system | E3 Ubiquitin ligase MARCH6 | IPI00105518 | 29 | |
| UPS system | Proteasome subunit alpha type-5 | IPI00291922 | 23 | |
| antioxidant | Glutathione S-transferase | IPI00219757 | 60 | |
| antioxidant | Thioredoxin | IPI00216298 | 71 | |
| mitochondrial maintenance | Prohibitin-1 [22] | IPI00017334 | 38 | |
| mitochondrial maintenance | Prohibitin-2 [22] | IPI00027252 | 54 | |
| microtubule remodeling | Stathmin | IP00479997 | 37 | |
| neurite growth | Neuron Navigator 2 isoform 1 | IPI00217052 | 27 | |
| antinflammatory | Cytosolic Phosolipase A2 | IPI00384577 | 62 | |
| mitochondrial apoptosis | Porin/VDAC1 | IPI00216308 | 48 | |
| mitochondrial apoptosis | Porin/VDAC2 | IPI00241145 | 54 | |
| 100nM | chaperone/foldase | HSP90-beta [5,14] | IPI00414676 | 170 |
| chaperone/foldase | HSP90-alpha isoform 2 [5,14] | IPI00382470 | 52 | |
| chaperone/foldase | HSP90AB2P [5,14] | IPI00455599 | 49 | |
| chaperone/foldase | HSP90B1 endoplasmin/GRP94 [5,14] | IPI00027230 | 59 | |
| chaperone/foldase | HSP90 ATPase activator [5,14] | IPI00030706 | 53 | |
| chaperone/foldase | BiP | IPI00003362 | 101 | |
| chaperone/foldase | HSP70 protein 1 [25] | IPI00304925 | 93 | |
| chaperone/foldase | HSP70 protein 4 [25] | IPI00002966 | 53 | |
| chaperone/foldase | HSP70 protein 6 [25] | IPI00339269 | 93 | |
| chaperone/foldase | HSC71 isoform 1 [25] | IPI00003865 | 236 | |
| chaperone/foldase | GRP75 | IPI00007765 | 76 | |
| chaperone/foldase | HSP60 | IPI00917575 | 235 | |
| chaperone/foldase | HSP70/HSP90 organizing protein [3] | IPI00013894 | 33 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit beta [38] | IPI00297779 | 94 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit delta [38] | IPI00302927 | 67 | |
| chaperone/foldase | TCP1 T-complex protein 1 subunit epsilon [38] | IPI00010720 | 109 | |
| chaperone/foldase | Peptidyl-prolyl cis-trans isomerase B | IPI00646304 | 72 | |
| chaperone/foldase | Protein disulfide-isomerase A3 [36] | IPI00025252 | 59 | |
| chaperone/foldase | Protein disulfide-isomerase A6 isoform 2 [36] | IPI00299571 | 107 | |
| chaperone/foldase | Calnexin | IPI00020984 | 52 | |
| UPS system | Ubiquitin carboxyl-terminal hydrolase isozyme L1 | IPI00018352 | 56 | |
| UPS system | Ubiquitin-like modifier-activating enzyme 1 | IPI00645078 | 38 | |
| UPS system | Proteasome subunit beta type-1 | IPI00025019 | 30 | |
| UPS system | Proteasome subunit beta type-6 | IPI00000811 | 44 | |
| antioxidant | Glutathione S-transferase | IPI00219757 | 80 | |
| antioxidant | Thioredoxin | IPI00216298 | 57 | |
| microtubule remodeling | Stathmin | IP00479997 | 77 | |
| microtubule remodeling | Stathmin-2 | IPI00218667 | 65 | |
| mitochondrial maintenance | Prohibitin-1 [22] | IPI00017334 | 60 | |
| mitochondrial maintenance | Prohibitin-2 [22] | IPI00027252 | 70 | |
| export misfolded proteins | Transitional ER ATPase | IPI00022774 | 46 | |
| suppress apoptosis | Apoptosis Inhibitor 5 isoform 2 | IPI00554742 | 32 | |
| pro-apoptosis | Cytochrome c | IPI00465315 | 34 |