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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Nutr Biochem. 2010 Jul 22;22(5):450–458. doi: 10.1016/j.jnutbio.2010.03.014

Figure 1. Curcumin treatment suppresses cytokine release in HG-induced THP-1cells.

Figure 1

Human monocytic (THP-1) cells(1 × 105 cells/ml) were cultured in presence of osmolar control (19.5 mmol/L mannitol) or normal glycemic (NG, 5.5 mmol/L glucose ) or hyperglycemic ( HG, 25 mmol/L) conditions in absence or presence of curcumin for 72 h as described in Methods. After that the media was saved. Cytokines were measured with a Milliplex ™ MAP Assay Kit (Millipore,USA)) according to the manufacturer's instructions. Values were calculated based on a standard curve constructed for the assay. Results were shown as mean ± SD of 5 different experiments. *a: p<0.05 compared to NG; *p<0.05; **p<0.01 compared to HG