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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Nutr Biochem. 2010 Jul 22;22(5):450–458. doi: 10.1016/j.jnutbio.2010.03.014

Figure 7. Effect of curcumin on chromatin events at the promoters of inflammatory genes.

Figure 7

ChIP assays were performed using Chip-IT™ Express (Active Motif) according to the manufacturer's instructions. Immunoprecipitations were performed overnight at 4°C with 5 μL of p300 antibody. DNA was subjected to PCR. ChIP assays showed the recruitment of p300 to the TNF-α (A) and IL-6 (B) promoters. Results of 1 typical experiment of 3 are shown. Values from ChIP with anti-p300 antibody represents the fold difference relative to those from IgG control antibody. Data are the average of three independent experiments each, and error bars represent standard deviations. *a: p<0.05 compared to NG; *p<0.05 compared to HG..