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. Author manuscript; available in PMC: 2012 Jan 2.
Published in final edited form as: DNA Repair (Amst). 2010 Oct 16;10(1):56–64. doi: 10.1016/j.dnarep.2010.09.007

Figure 3.

Figure 3

The kinetics of dCTP incorporation opposite Gua and N2-ethyl-Gua were measured in a rapid kinetic experiment under enzyme limiting conditions (50 nM DNA pol κ, 100 nM template-primer DNA, and 8 mM MgCl2 or MnCl2). The data were fit to the burst equation (see Materials and Methods) and the following kinetic parameters were determined: opposite template Gua (A) the kobs = 3.5 ± 0.3 s−1; kss = 4.4 ± 1.0 s−1; A = 63 ± 3 nM in the presence of MgCl2 and using MnCl2 the kobs = 1.4 ± 0.2 s−1; kss = 3.8 ± 1.0 s−1; A = 47 ± 5 nM; opposite template N2-ethyl-Gua (B) the kobs = 4.0 ± 0.6 s−1; kss = 4.7 ± 1.4 s−1; A = 62 ± 4 nM in the presence of MgCl2 and using MnCl2 the kobs = 2.1 ± 0.3 s−1; kss = 7 ± 1 s−1; A = 40 ± 4 nM.