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Determination of KddCTP for DNA pol κ in the presence of MgCl2 (A) or MnCl2 (B). DNA pol κ (80 nM) was incubated with 100 nM template-primer DNA and mixed with increasing dCTP (50–750 μM) concentrations. A plot of “burst” rates (s−1) versus dCTP concentration was fit to a hyperbolic equation to determine the maximum rate (kpol) and the dissociation constant (Kd) for dCTP incorporation.
