Table 1.

Effect of ip Ucn 2 on 4-h meal pattern of light phase feeding in overnight fasted/re-fed mice or dark phase feeding in ad libitum fed mice

Parameter	Overnight fasted/re-fed in light phase		Fedad libitumin dark phase
	Vehicle	Ucn 2 (10 µg/kg)	Vehicle	Ucn 2 (10 µg/kg)	Vehicle	Ucn 2 (30 µg/kg)
Meal frequency (number/4h)	2.38 ± 0.56	4.25 ± 0.56*	4.86 ± 0.74	4.86 ± 0.67	4.86 ± 0.67	4.13 ± 0.29
Meal size (g/meal)	0.82 ± 0.12	0.24 ± 0.03***	0.14 ± 0.01	0.13 ± 0.02	0.17 ± 0.01	0.10 ± 0.01***
Meal duration (min)	74.49 ± 14.88	22.56 ± 2.93**	16.67 ± 2.70	14.86 ± 1.94	16.84 ± 1.20	11.32 ± 0.77**
Feeding bouts (number/4h)	25.88 ± 2.24	21.38 ± 2.50	22.14 ± 5.82	26.00 ± 6.42	22.57 ± 2.75	18.75 ± 1.96
Total meal time (min/4h)	130.18 ± 6.53	88.24 ± 9.34**	84.85 ± 19.38	72.50 ± 13.28	77.91 ± 6.66	45.80 ± 3.29***
Inter-meal interval (min)	126.54 ± 23.85	56.45 ± 6.91*	29.66 ± 6.30	33.96 ± 4.22	41.34 ± 6.32	40.23 ± 3.65
Time spent in meals (%/4h)	54.24 ± 2.72	36.76 ± 3.89**	35.35 ± 8.08	30.21 ± 5.54	32.46 ± 2.78	19.08 ± 1.37***
Latency to 1st meal (min)	4.29 ± 0.81	5.38 ± 1.15	33.67 ± 13.36	44.70 ± 19.31	14.38 ± 8.14	68.34 ± 16.17*
Duration 1st meal (min)	87.38 ± 12.93	15.56 ± 3.33***	11.94 ± 3.00	11.58 ± 3.54	16.69 ± 3.19	8.48 ± 1.89*
Eating rate 1st meal (mg/min)	7.27 ± 1.59	4.77 ± 0.82	3.61 ± 1.28	3.31 ± 0.88	2.69 ± 0.65	1.74 ± 0.61
Eating rate/4h (mg/min)	6.61 ± 1.48	4.01 ± 0.49	3.63 ± 0.56	3.08 ± 0.17	3.32 ± 0.42	2.33 ± 0.17*
Satiety ratio (min/g)	156.53 ± 14.20	239.54 ± 29.70*	253.80 ± 74.03	282.09 ± 31.62	244.01 ± 33.79	419.12 ± 30.32**

Each group had vehicle controls tested at the same time of Ucn 2 and also injected ip. Data are mean ± SEM; n=7–8 mice/group.

^*p < 0.05,

^**< 0.01,

^***< 0.001 vs. vehicle, respectively.