Figure 4.
Effect of apocynin on recruitment of cytosolic subunits to the cell membrane. (A) Western blot analysis of the membrane fraction 6 hours after transient global cerebral ischemia (tGCI) showed that apocynin treatment significantly attenuated recruitment of cytosolic subunits p47phox and p67phox to the membrane compared with vehicle treatment (n=4, *P<0.05). Na-K ATPase was used as an internal control. (B) An immunofluorescent study using a confocal microscope also showed the effect of apocynin treatment. Microtubule-associated protein-2 (MAP2) immunostaining demarcates the neuronal cytoplasmic space. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Apo, apocynin; Veh, vehicle. Scale bar=20 μm.