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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2010 Jul 12;80(3):705–711. doi: 10.1016/j.ijrobp.2010.02.063

Table 1.

Specific antibodies to evaluate NF-κB activation.

Target Ab Manufacturer Catalog No. Dilution Positive Control Tissue
P50 p50NLS Santa Cruz sc-114NLS 1:100 Breast Ca, Panc-1 cell block*
P65 F-6 Santa Cruz sc-8008 1:1000 Tonsil, Panc-1 cell block*
P52 c-5 Santa Cruz sc-7386 1:50 Tonsil, Panc-1 cell block*
*

Panc-1 is a pancreatic cancer cell line stained before and after treatment with TNF-α that increases nuclear p50 and p65. A positive control has baseline staining that increases significantly after TNF treatment.