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. 2010 Dec;91(6):506–514. doi: 10.1111/j.1365-2613.2010.00734.x

Figure 3.

Figure 3

DNA damage mediated increase in MDC1 expression is compromised in STAT3−/− MEFs. (a) Basal MDC1 expression was measured in STAT3+/+ and STAT3−/− MEFs by qPCR. (b) Wild type MEFs were treated with 10μM etoposide for the indicated times and MDC1 levels were measured by qPCR. (c) STAT3+/+ and STAT3−/− MEFs were treated for 12 h with 10μM etoposide and MDC1 levels were measured by qPCR. (d) Wild type MEFs were irradiated with 10 Gy γ and MDC1 levels were measured at indicated time points after treatment. (e) STAT3+/+ and STAT3−/− MEFs were treated for 8 h with 10 Gy γ-irradiation and MDC1 levels were measured by qPCR. Error bars represent mean ± SEM, Statistical analysis was carried out using a student's t-test (a), one-way anova with Dunnett's post test (b, d) or a two-way anova with Bonfferoni post test (c, e), n = 2 per experiment, repeated in triplicate. *P < 0.05, **P < 0.01.