Western blot analysis of the effect of antisense knock-down (AS-ODNs) of PI3Kδ on the expression of PI3Kα (A), PI3Kβ (B) and PI3Kγ (C) isoforms in aortas from normoglycaemic (control) and diabetic mice. On the left are shown the mean ± SEM values of three different experiments, and on the right representative gels. Results were normalized by β-actin content in samples. Control MM-ODN versus diabetic MM-ODN ***P < 0.001, **P < 0.01. AS-ODNs, antisense oligodeoxynucleotides; MM-ODNs, mismatch oligodeoxynucleotides; PI3K, phosphatidylinositol 3-kinase.