Figure 7.

IL-6 expression was increased after cholesterol replenishment in human umbilical vein endothelial cells treated with emodin and methyl-β-cyclodextrin (MBCD). Cells were treated with emodin (30 and 50 µg·mL⁻¹), MBCD (10 mM) or culture medium alone for 60 min, replaced by fresh cell culture medium, and followed by cholesterol (cho) replenishment (80 µg·mL⁻¹) or not for 60 min. The cells were then stimulated with 0.1 µg·mL⁻¹ lipopolysaccharide for 4 h. Cells treated with cholesterol alone for 4 h served as another control. IL-6 expression was detected by elisa.