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. 2010 Dec;161(7):1645–1660. doi: 10.1111/j.1476-5381.2010.00977.x

Figure 2.

Figure 2

Stimulation of PDE2 by the GAF domain ligands 5,6-DM-cBIMP and 5,6-DCI-cBIMP. PDE activity was measured in cytosolic fractions of PDE2 overexpressing HEK cells in the presence of increasing concentrations of 5,6-DM-cBIMP (A, D), 5,6-DCI-cBIMP (B, E) or cGMP (C) at the indicated concentrations of cAMP (A–C) and cGMP (D–E) as substrate. The reactions were carried out in 5 min incubations as described in detail in Methods. Data shown are mean ± SD of at least three independent experiments performed in duplicate.