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. 2010 Dec;161(8):1778–1792. doi: 10.1111/j.1476-5381.2010.01003.x

Figure 7.

Figure 7

Effects of arginine vasopressin (AVP) upon the phosphorylation of Thr346/356/366 in protein encoded by n-myc downstream regulated gene 1 (NDRG1) in control, TORIN1-treated and PP242-treated cells. (A) Typical Western blots showing the effects of AVP (10 nM, 30 min exposure) upon the abundance of the Thr346/356/366-phosphorylated (upper panels) and total NDRG1 (lower panels) in control (Cont.) and TORIN1-pretreated (0.1 µM, 30 min) cells. (B) Pooled data showing the effects of AVP upon the abundance of Thr346/356/366-phosphorylated NDRG1 in control and TORIN1-treated cells (n = 6). (C) Western blots showing the effects of AVP (10 nM, 30 min exposure) upon the abundance of the Thr346/356/366-phosphorylated (upper panels) and total NDRG1 (lower panels) in control and PP242-pretreated (1 µM, 30 min) cells. (D) Pooled data showing the effects of AVP upon NDRG1-Thr346/356/366 phosphorylation in control and PP242-treated cells (n = 6). *P < 0.05, significant effects of AVP; Student's paired t-test. †P < 0.05, significant effects of TORIN1 or PP242; one-way anova, Bonferroni post hoc test). Unstim., unstimulated.