Figure 1.

Chromatograms of purines obtained by reverse-phase HPLC. A standard mixture (2.0 pmol) of ATP, ADP, AMP and adenosine was injected with internal standard (IS) after ethenopurine derivatization and monitored with fluorescence detector (A). A standard solution of inosine (2.0 pmol) was injected with IS and monitored with UV detector (B). Sample solutions collected 10 min after incubation of isolated spinal cord with normoxic or hypoxic ACSF were injected and monitored with fluorescence detector (C) and UV detector (D). Superimposed chromatograms in normoxia and hypoxia were normalized to the peak amplitude of IS.