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. 2010 Dec 21;2011:307631. doi: 10.1155/2011/307631

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Copyright © 2011 Kerry J. Welsh et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comparison of immunohistochemistry (IHC) for MTB and Zhiel-Neelsen acid fast staining. Early lipid pneumonia (a)–(c). (a) H&E stain demonstrates macrophages and lymphocytes within the alveoli, 100x. (b) MTB are noted on the IHC, 400x. (c) Few AFB are observed by Zhiel-Neelsen staining, 400x. Lipid pneumonia undergoing abrupt necrosis (d)–(f). (d) Cells in alveoli undergo necrosis and have a homogenous appearance, 100x. (e) Abundant mycobacterial antigens noted on the IHC stain for MTB, 400x. (f) Numerous acid-fast bacilli are observed, 400x. Cavitary TB (g)–(i). (g) H&E stain showing a cavity with a piece of detached lung and necrotic debris, 40x. (h) The wall of the cavity is strongly positive for MTB antigens, 400x. (i) Acid-fast bacilli are found lining the cavity wall, 400x.