Table 1.
Marker | Pretreatment | Dilution | Incubation timea |
---|---|---|---|
CD4 | steam for 20 minutes in EDTA, pH 8.0 | 1 : 25 | 45 |
CD8 | steam for 20 minutes in EDTA, pH 8.0 | 1 : 125 | 30 |
CD20 | 5 minutes under pressure in citrate buffer, pH 6.0 | 1 : 800 | 20 |
CD31 | 5 minutes under pressure in citrate buffer, pH 6.0 | 1 : 100 | 30 |
CD68 | 5 minutes under pressure in citrate buffer, pH 6.0 | 1 : 2000 | 20 |
DEC-205 | steam for 20 minutes in EDTA, pH 8.0 | 1 : 50 | overnight |
Foxp3 | 5 minutes under pressure in citrate buffer, pH 6.0 | 1 : 100 | 30 |
MTB | Proteinase K | 1 : 100 | 60 |
aminutes.