Table 2.
Variant specificity.
| LoxP spacer pairs | Inversion | Deletion | wt |
|---|---|---|---|
| 171 bp | 240 bp | 360 bp | |
| LoxP-LoxP | 51.7% | 48.3% | 0.0% |
| Loxm2-Loxm2 | 0.0% | 99.3% | 0.7% |
| LoxP-Loxm2 | 49.0% | 0.7% | 50.3% |
| Loxm2-Lox2722 | 0.0% | 0.0% | 100.0% |
| Loxm2-Lox5171 | 0.0% | 0.0% | 100.0% |
| Lox2722-LoxP | 0.0% | 0.0% | 100.0% |
| LoxP-Lox5171 | 55.2% | 0.0% | 44.8% |
| Lox2722-Lox5171 | 0.0% | 0.0% | 100.0% |
The percentage of clones of each type product (inversion, deletion, or WT) present in a PCR band is indicated Lox spacer pairs. Inversion indicates a reaction involving the LoxP site in the pBelo BAC backbone (inverted spacer repeat) while deletions indicate a reaction between the indicated Lox pair (direct spacer repeats). Percentages are based on positives out of 144 samples.