TABLE 3. Effect of chronic treatment with TRH on acute antagonism of pentobarbital-induced sleep^a.

Pretreatmentb	Acute TRHb Dose	Sleeping Time	N
	mg/kg	min ± S.E.M.
Control (saline)		63.0 ± 4.32	10
Chronic TRH	1	28.5 ± 3.2c	13
Chronic TRH	5	21.2 ± 3.3c	13
Saline	1	21.2 ± 5.5c	10
Saline	5	24.0 ± 5.4c	10

^aAll mice were give pentobarbital sodium (55 mg/kg).

^bTRH (20 mg/kg) was administered to mice two times daily for 3 days with an additional dose of TRH (20 mg/kg) being given 4 hours prior to the acute administration of 1 or 5 mg/kg of TRH which was administered 1 minute before the pentobarbital sodium solution.

^cP < .001 when compared with saline-treated control.