Figure 2.

Histograms of bond lifetimes for the αIIbβ3-fibrinogen interactions measured under a constant tensile force of f_ext = 50 pN and contact duration (interaction) T = 0.5 s. The bond lifetimes in the absence (A) and presence of 200 μg/ml abciximab, a specific αIIbβ3 antagonist (B), or 1 mM Mn²⁺, an integrin activator (C), are compared with the theoretically derived probability density functions of bond lifetimes (fitting curves) obtained using Eq. 6. In the histograms, the bin size is obtained using an adaptive approach to the optimal bandwidth selection (52). (Insets) Raw data traces typical for each experimental condition represented in the figure.