Table 2.
Average forced unbinding characteristics from the Brownian oscillator model for the weak (LR1) and strong (LR2) bound states of the αIIbβ3-fibrinogen complex
| Control |
With abciximab |
With Mn2+ |
||||
|---|---|---|---|---|---|---|
| LR1 | LR2 | LR1 | LR2 | LR1 | LR2 | |
| Populations, λ1 and λ2 | 0.83 (0.04) | 0.17 (0.04) | 0.97 (0.03) | 0.03 (0.03) | 0.71 (0.03) | 0.29 (0.03) |
| Unbinding rate constants, ku1 and ku2 (s−1) | 3.06 (0.11) | 0.25 (0.01) | 3.38 (0.33) | 0.25 (0.01) | 2.84 (0.17) | 0.189 (0.002) |
| Spring constant, ksp (pN/nm) | — | 12.00 (0.07) | — | 12.02 (0.08) | — | 13.58 (0.08) |
| Critical extension, x∗ (nm) | — | 2.46 (0.05) | — | 2.52 (0.04) | — | 2.86 (0.05) |
| Unbinding energy, kBT | — | 8.78 (0.41) | — | 9.20 (0.28) | — | 12.42 (0.53) |
Analytical estimates of the mechanical, kinetic, and equilibrium parameters correspond to a tensile force of fext = 50 pN. The model and parameters are introduced in the Materials and Methods section. Numbers in parentheses are standard deviations.