Table 3.
Kinetic and thermodynamic characteristics for the weak (LR1) and strong (LR2) bound states of the αIIbβ3-fibrinogen complex at different tensile forces (fext)
|
fext = 50 pN |
fext = 20–40 pN |
fext = 10–20 pN |
fext = 0 pN∗ |
|||||
|---|---|---|---|---|---|---|---|---|
| LR1 | LR2 | LR1 | LR2 | LR1 | LR2 | LR1 | LR2 | |
| Populations, λ1 and λ2 | 0.83 (0.04) | 0.17 (0.04) | 0.72 (0.03) | 0.28 (0.03) | 0.62 (0.02) | 0.38 (0.02) | 0.58 | 0.42 |
| Unbinding rate constants, ku1 and ku2 (s−1) | 3.06 (0.11) | 0.25 (0.01) | 1.64 (0.11) | 0.207 (0.008) | 1.28 (0.08) | 0.16 (0.01) | 1.1 | 0.15 |
| Spring constant, ksp (pN/nm) | — | 12.00 (0.07) | — | 9.30 (0.45) | — | 8.00 (0.71) | — | 7.5 |
| Critical extension, x∗ (nm) | — | 2.46 (0.05) | — | 2.26 (0.09) | — | 1.98 (0.13) | — | 1.78 |
| Unbinding energy, kBT | — | 8.78 (0.41) | — | 5.73 (0.37) | — | 3.78 (0.41) | — | 2.9 |
Parameters are introduced in the Materials and Methods section. Numbers in parentheses are standard deviations.
∗
The parameters for fext = 0 pN were determined by linear extrapolation.