Figure 1.

(a) Signaling pathways in platelets converge on intracellular calcium release. (b) High throughput experimental procedure. An agonist plate containing combinatorial agonist combinations and a platelet plate containing dye loaded platelets were separately assembled. Agonists were dispensed onto platelet suspensions and fluorescence changes were measured to quantify platelet calcium concentrations [Ca²⁺]_i. [Ca²⁺]_i transients can be represented as overlapping plots (right) or parallel heat maps (left). (c) Dynamic neural network used to train platelet response to combinatorial agonist activation. A constant sequence of input signals (agonist concentrations) is introduced to the 2-layer, 12-node network at each time point. Processing layers integrate input values with feedback signals to predict [Ca²⁺]_i at the next time point.