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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Nat Biotechnol. 2010 Jun 20;28(7):727–732. doi: 10.1038/nbt.1642

Figure 2.

Figure 2

(a) Pairwise agonist scanning (PAS) All 154 binary combinations of the agonists CVX, ADP, U46619, SFLLRN, AYPGKF and PGE2 at concentrations of 0, 0.1, 1 and 10x EC50 were combined on the same plate (in replicates of 2) and the dynamic response of the platelet to each combination was recorded. The NN model was trained on this dataset. (b) Pairwise agonist synergy scores, which reflect the gain or loss in calcium response due to agonist cross talk, were calculated for both experimental and predicted time course traces. EC50: PGE2, 24.6 μM; AYPGKF, 112 μM; SFLLRN, 15.2 μM; U46619, 1.19 μM; ADP, 1.17 μM; CVX, 0.00534 μM.