Figure 3. NN model reveals the global platelet response to all agonist combinations.

(a) Measurement and prediction of the platelet response to all 64 ternary combinations of ADP, SFLLRN and CVX at 0, 0.1, 1, and 10 × EC₅₀. The NN model was trained only on pairwise interactions but successfully predicted ternary interactions. (b) Measurement and prediction of the platelet response to 45 predictions in the full combinatorial agonist space (see text). (c) Predicted versus measured synergy scores for the 45 conditions in panel b. (right) Distribution of synergy scores for all 4077 possible experimental conditions. (bottom) Experimental conditions for the 45 sampled combinations of agonists, arranged in order of increasing synergy. The orange bar denotes the 3 most highly synergistic conditions, which all contained high U46619, no PGE₂ and low levels of other agonists. (d) Measured and predicted platelet responses to sequential additions of ADP, SFLLRN, and CVX.