Fig. 2.

Human peripheral blood mononuclear cells (PBMC) stimulated with Cytostim for 3 h or unstimulated controls, assayed with the interleukin (IL)-17 secretion assay. (a) Stimulated and unstimulated cells pre- and post-isolation over two MS columns on a mini-MACS magnet. IL-17⁺ cells are >90% CD4⁺ CD154⁺. (b). Cells stained with CD4 and CD161 confirm that >90% of IL-17⁺ cells are CD161⁺. (c) Cells co-processed with interferon (IFN)-γ or IL-2 secretion assays show that there is a significant population of IL2⁺ IL-17⁺ cells (box) but not of IFN-γ⁺ IL-17+ cells. Note that IL-2 and IFN-γ are produced in significantly larger quantities than IL-17.