Fig. 3.
BALB/c spleen cells stimulated with phorbol myristate acetate (PMA) and ionomycin for 3–6 h, or unstimulated controls. (a) Interleukin (IL)-17 is secreted by CD4+, CD8+γ/δ T cell receptor (TCR)+ T cells and natural killer (NK) T cells at 3 h. (b) Kinetics of IL-17 secretion by CD4+ cells. The maximum percentage positive cells (•) and maximum mean fluorescence intensity (♦) are both reached within 3–4 h of stimulus. : Unstimulated controls.