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Analysis of interferon (IFN)-γ-dependent regulation of pro- and anti-apoptotic proteins in human colon epithelial cells (HT-29). HT-29 cells were cultured for indicated time with IFN-γ (100 ng/ml) or in serum-free medium. Using reverse transcription–polymerase chain reaction (RT–PCR) the influence of IFN-γ on the pro-apoptotic protein Bad and the anti-apoptotic protein Bcl-XL was examined. NTC: non-template control.
