Fig. 6.

Western blot analysis for detection of interferon (IFN)-γ-mediated signal pathways. Human colon epithelial cells (HT-29) were stimulated with IFN-γ (100 ng/ml) for 2–24 h. Consecutive phosphorylation of signal transducer and activator of transcription (STAT)-1 was detected using Western blot analysis. 24 h in serum-free medium cultured HT-29 cells were used as controls. Similar results were detectable in Caco-2 cells (see Fig. 8b).