Fig. 8.

Interferon (IFN)-γ-mediated interleukin (IL)-18 binding protein (bp) expression after inhibition of JAK tyrosine kinase. Caco-2 cells were cultured for 48 h with IFN-γ (100 ng/ml) and JAK tyrosine kinase inhibitor AG490 (40 and 80 µM) alone and in combination. Resulting IL-18bp expression was measured by enzyme-linked immunosorbent assay in cell supernatants (a) as well as in cell lysates (b). Caco-2 cells cultured for 48 h in serum-free medium were used as controls. Data represent the results from three independent experiments.