Fig. 4.

Involvement of intercellular adhesion molecule-1 (ICAM-1) in co-culture system. SW982 cells were cultured with interleukin (IL)-6 (100 ng/ml) + sIL-6R (100 ng/ml) for 24 h. The expression of ICAM-1 mRNA was measured with real-time polymerase chain reaction (PCR), and ICAM-1 expression on the cell surface was analysed by flow cytometry (a). RAW cells were co-cultured with SW982 cells in the presence of IL-6 + sIL-6R with or without anti-ICAM-1 antibody (10 µg/ml) for 24 h, and tartrate-resistant acid phosphatase isoform 5b (TRAP5b) mRNA expression was measured by real-time PCR (b). Statistical significances were analysed by t-test (*P < 0·05).