Fig. 5.

Intercellular adhesion molecule-1 (ICAM-1) reversed suppression of osteoclast differentiation of RAW cells by interleukin (IL)-6. RAW cells were stimulated by soluble receptor activator of nuclear factor-κB ligand (sRANKL) (50 ng/ml) in the presence of IL-6 (100 ng/ml) and sICAM-1 (10, 100, 1000 ng/ml) for 24 h (a). RAW cells were stimulated by sRANKL (50 ng/ml) in the presence of IL-6 (100 ng/ml) and sICAM-1 (1000 ng/ml) for 24 h (b). After culture, tartrate-resistant acid phosphatase isoform 5 (TRAP5) band nuclear factor of activated T cells cytoplasmic 1 (NFATc1) mRNA expression was measured by real-time polymerase chain reaction (PCR). Statistical significances were analysed by t-test (*P < 0·05).